subjectId	Discipline Name	Subject Name	Coordinators	Type	Institute	Content
102101003	Biotechnology	Biomathematics	Dr. Ranjith Padinhateeri	Video	IIT Bombay	Select L1-Introduction L2-Graphs and functions - I L3-Graphs and functions - II L4-Functions and derivatives L5-Calculation of derivatives L6-Differentiation and its application in Biology - I L7-Differentiation and its application in Biology - II L8-Differentiation and its application in Biology - III L9-Differentiation and its application in Biology - IV L10-Integration - I L11-Integration - II L12-Differential equations-I L13-Differential equations - II L14-Vectors - I L15-Vectors - II L16-Vectors - III L17-Nernst equation L18-Diffusion-I : Diffusion equation L19-Diffusion - II: Mean-square displacement L20-Diffusion-III : Einstein�s relation L21-Statistics : Mean and variance L22-Statistics: Distribution function L23-Understanding Normal distribution L24-Fitting a function to experimental data L25-Size of a flexible protein: Simplest model L26-Uniform and Poisson distributions; Knudson�s analysis L27-Fourier Series-I L28-Fourier Series-II L29-Fourier transform L30-Master equation: Polymerization dynamics, Molecular motor motion L31-Evolution: Simplest model L32-Tutorial - I L33-Tutorial-II L34-Temperature, Energy and Entropy L35-Partition function, Free energy L36-Bending fluctuations of DNA and spring-like proteins L37-Force-extension and looping of DNA L38-Thermodynamics of protein organization along DNA L39-Learning mathematics with the help of a computer
102101007	Biotechnology	Proteomics: Principles and Techniques	Prof. Sanjeeva Srivastava	Video	IIT Bombay	Select VIDEO-L1 PPT-L1 HANDOUT-L1 QUIZ-L1 ASSIGNMENTS-L1 TRANSCRIPT-L1 VIDEO-L2 PPT-L2 HANDOUT-L2 QUIZ-L2 ASSIGNMENTS-L2 TRANSCRIPT-L2 VIDEO-L3 PPT-L3 HANDOUT-L3 QUIZ-L3 ASSIGNMENTS-L3 TRANSCRIPT-L3 VIDEO-L4 PPT-L4 HANDOUT-L4 QUIZ-L4 ASSIGNMENTS-L4 TRANSCRIPT-L4 VIDEO-L5 PPT-L5 HANDOUT-L5 QUIZ-L5 ASSIGNMENTS-L5 TRANSCRIPT-L5 VIDEO-L6 PPT-L6 HANDOUT-L6 QUIZ-L6 ASSIGNMENTS-L6 TRANSCRIPT-L6 VIDEO-L7 PPT-L7 HANDOUT-L7 QUIZ-L7 ASSIGNMENTS-L7 TRANSCRIPT-L7 VIDEO-L8 PPT-L8 HANDOUT-L8 QUIZ-L8 ASSIGNMENTS-L8 TRANSCRIPT-L8 VIDEO-L9 PPT-L9 HANDOUT-L9 QUIZ-L9 ASSIGNMENTS-L9 TRANSCRIPT-L9 VIDEO-L10 PPT-L10 HANDOUT-L10 QUIZ-L10 ASSIGNMENTS-L10 TRANSCRIPT-L10 VIDEO-L11 PPT-L11 HANDOUT-L11 QUIZ-L11 ASSIGNMENTS-L11 TRANSCRIPT-L11 VIDEO-L12 PPT-L12 HANDOUT-L12 QUIZ-L12 ASSIGNMENTS-L12 TRANSCRIPT-L12 VIDEO-L13 PPT-L13 HANDOUT-L13 QUIZ-L13 ASSIGNMENTS-L13 TRANSCRIPT-L13 VIDEO-L14 PPT-L14 HANDOUT-L14 QUIZ-L14 ASSIGNMENTS-L14 TRANSCRIPT-L14 VIDEO-L15 PPT-L15 HANDOUT-L15 QUIZ-L15 ASSIGNMENTS-L15 TRANSCRIPT-L15 VIDEO-L16 PPT-L16 HANDOUT-L16 QUIZ-L16 ASSIGNMENTS-L16 TRANSCRIPT-L16 VIDEO-L17 PPT-L17 HANDOUT-L17 QUIZ-L17 ASSIGNMENTS-L17 TRANSCRIPT-L17 VIDEO-L18 PPT-L18 HANDOUT-L18 QUIZ-L18 ASSIGNMENTS-L18 TRANSCRIPT-L18 VIDEO-L19 PPT-L19 HANDOUT-L19 QUIZ-L19 ASSIGNMENTS-L19 TRANSCRIPT-L19 VIDEO-L20 PPT-L20 HANDOUT-L20 QUIZ-L20 ASSIGNMENTS-L20 TRANSCRIPT-L20 VIDEO-L21 PPT-L21 HANDOUT-L21 QUIZ-L21 ASSIGNMENTS-L21 TRANSCRIPT-L21 VIDEO-L22 PPT-L22 HANDOUT-L22 QUIZ-L22 ASSIGNMENTS-L22 TRANSCRIPT-L22 VIDEO-L23 PPT-L23 HANDOUT-L23 QUIZ-L23 ASSIGNMENTS-L23 TRANSCRIPT-L23 VIDEO-L24 PPT-L24 HANDOUT-L24 QUIZ-L24 ASSIGNMENTS-L24 TRANSCRIPT-L24 VIDEO-L25 PPT-L25 HANDOUT-L25 QUIZ-L25 ASSIGNMENTS-L25 TRANSCRIPT-L25 VIDEO-L26 PPT-L26 HANDOUT-L26 QUIZ-L26 ASSIGNMENTS-L26 TRANSCRIPT-L26 VIDEO-L27 PPT-L27 HANDOUT-L27 QUIZ-L27 ASSIGNMENTS-L27 TRANSCRIPT-L27 VIDEO-L28 PPT-L28 HANDOUT-L28 QUIZ-L28 ASSIGNMENTS-L28 TRANSCRIPT-L28 VIDEO-L29 PPT-L29 HANDOUT-L29 QUIZ-L29 ASSIGNMENTS-L29 TRANSCRIPT-L29 VIDEO-L30 PPT-L30 HANDOUT-L30 QUIZ-L30 ASSIGNMENTS-L30 TRANSCRIPT-L30 VIDEO-L31 PPT-L31 HANDOUT-L31 QUIZ-L31 ASSIGNMENTS-L31 TRANSCRIPT-L31 VIDEO-L32 PPT-L32 HANDOUT-L32 QUIZ-L32 ASSIGNMENTS-L32 TRANSCRIPT-L32 VIDEO-L33 PPT-L33 HANDOUT-L33 QUIZ-L33 ASSIGNMENTS-L33 TRANSCRIPT-L33 VIDEO-L34 PPT-L34 HANDOUT-L34 QUIZ-L34 ASSIGNMENTS-L34 TRANSCRIPT-L34 VIDEO-L35 PPT-L35 HANDOUT-L35 QUIZ-L35 ASSIGNMENTS-L35 TRANSCRIPT-L35 VIDEO-L36 PPT-L36 HANDOUT-L36 QUIZ-L36 ASSIGNMENTS-L36 TRANSCRIPT-L36 VIDEO-L37 PPT-L37 HANDOUT-L37 QUIZ-L37 ASSIGNMENTS-L37 TRANSCRIPT-L37 VIDEO-L38 PPT-L38 HANDOUT-L38 QUIZ-L38 ASSIGNMENTS-L38 TRANSCRIPT-L38 VIDEO-L39 PPT-L39 HANDOUT-L39 QUIZ-L39 ASSIGNMENTS-L39 TRANSCRIPT-L39 VIDEO-L40 PPT-L40 HANDOUT-L40 QUIZ-L40 ASSIGNMENTS-L40 TRANSCRIPT-L40
102101040	Biotechnology	Advanced Clinical Proteomics	Prof. Sanjeeva Srivastava	Web	IIT Bombay	Select LECTURE-L-1 QUIZ-L-1 ASSIGNMENT-L-1 HANDOUT-L-1 LECTURE-L-2 QUIZ-L-2 ASSIGNMENTS-L-2 HANDOUT-L-2 LECTURE-L-3 QUIZ-L-3 ASSIGNMENTS-L-3 HANDOUT-L-3 LECTURE-L-4 QUIZ-L-4 ASSIGNMENTS-L-4 HANDOUT-L-4 LECTURE-L-5 QUIZ-L-5 ASSIGNMENTS-L-5 HANDOUT-L-5 LECTURE-L-6 QUIZ-L-6 ASSIGNMENTS-L-6 HANDOUT-L-6 LECTURE-L-7 QUIZ-L-7 ASSIGNMENTS-L-7 HANDOUT-L-7 LECTURE-L-8 QUIZ-L-8 ASSIGNMENTS-L-8 HANDOUT-L-8 LECTURE-L--9 QUIZ-L-9 ASSIGNMENTS-L-9 HANDOUT-L-9 LECTURE-L-10 QUIZ-L-10 ASSIGNMENTS-L-10 HANDOUT-L10 LECTURE-L-11 QUIZ-L-11 ASSIGNMENTS-L-11 HANDOUT-L-11 LECTURE-L-12 QUIZ-L-12 ASSIGNMENTS-L-12 HANDOUT-L-12 LECTURE-L-13 QUIZ-L-13 ASSIGNMENTS-L-13 HANDOUT-L-13 LECTURE-L-14 QUIZ-L-14 ASSIGNMENT-L-14 HANDOUT-L-14 LECTURE-L-15 QUIZ-L-15 ASSIGNMENTS-L-15 HANDOUT-L-15 LECTURE-L-16 QUIZ-L-16 ASSIGNMENTS-L-16 HANDOUT-L-16 LECTURE-L-17 QUIZ-L-17 ASSIGNMENTS-L-17 HANDOUT-L-17 LECTURE-L-18 QUIZ-L-18 ASSIGNMENTS-L-18 HANDOUT-L-18 LECTURE-L-19 QUIZ-L-19 ASSIGNMENTS-L-19 HANDOUT-L-19 LECTURE-L-20 QUIZ-L-20 ASSIGNMENTS-L-20 HANDOUT-L-20 LECTURE-L-21 QUIZ-L-21 ASSIGNMENT-L-21 HANDOUT-L-21 LECTURE-L-22 QUIZ-L-22 ASSIGNMENT-L-22 HANDOUT-L-22 LECTURE-L-23 QUIZ-L-23 ASSIGNMENT-L-23 HANDOUT-L-23 LECTURE-L-24 QUIZ-L-24 ASSIGNMENT-L-24 HANDOUT-L-24 LECTURE-L-25 QUIZ-L-25 ASSIGNMENT-L-25 HANDOUT-L-25 LECTURE-L-26 QUIZ-L-26 ASSIGNMENT-L-26 HANDOUT-L-26 LECTURE-L-27 QUIZ-L-27 ASSIGNMENT-L-27 HANDOUT-L-27 LECTURE-L-28 QUIZ-L-28 ASSIGNMENT-L-28 HANDOUT-L-28 LECTURE-L-29 QUIZ-L-29 ASSIGNMENT-L-29 HANDOUT-L-29 LECTURE-L-30 QUIZ-L-30 ASSIGNMENT-L-30 HANDOUT-L-30 LECTURE-L-31 QUIZ-L-31 ASSIGNMENT-L-31 HANDOUT-L-31 LECTURE-L-32 QUIZ-L-32 ASSIGNMENT-L-32 HANDOUT-L-32 LECTURE-L-33 QUIZ-L-33 ASSIGNMENT-L-33 HANDOUT-L-33 LECTURE-L-34 QUIZ-L-34 ASSIGNMENT-L-34 HANDOUT-L-34 LECTURE-L-35 QUIZ-L-35 ASSIGNMENT-L-35 HANDOUT-L-35 LECTURE-L-36 QUIZ-L-36 ASSIGNMENT-L-36 HANDOUT-L-36 LECTURE-L-37 QUIZ-L-37 ASSIGNMENT-L-37 HANDOUT-L-37 LECTURE-L-38 QUIZ-L-38 ASSIGNMENT-L-38 HANDOUT-L-38 LECTURE-L-39 QUIZ-L-39 ASSIGNMENT-L-39 HANDOUT-L-39 LECTURE-L-40 QUIZ-L-40 ASSIGNMENT-L-40 HANDOUT-L-40
102102033	Biotechnology	Enzyme Science and Engineering	Prof. Subhash Chand	Video	IIT Delhi	Select Introduction and Scope to Enzyme Science & Engineering Characteristic Features of Enzymes Enzymes as Biocatalysts Enzymatic Catalysis Specificity of Enzyme Action Kinetics of Enzyme Catalyzed Reactions Kinetics of Enzyme Catalyzed Reactions Deviation from Hyperbolic Enzyme Kinetics Role of Effector Molecules in Enzyme Kinetics Reversible Inhibition Effect of PH and Temperature on Enzyme Kinetics of Bi substrate Enzyme Kinetics of Bi substrate Enzyme Immobilized Enzymes - I Immobilized Enzymes - II Immobilized Enzymes - III Immobilization of Enzymes by Entrapment Effect of Immobilization Reactors for Enzyme Catalyzed Reactions Idealized Enzyme Reactor Performance Idealized Enzyme Reactor Performance Kinetic Parameters for IME Systems Steady State Analysis of Mass Transfer Steady State Analysis of Mass Transfer Non Ideal Flow in Continuous Immobilized Enzyme Applications of Immobilized Enzymes in Process Analytical Applications Enzyme Technology Challenges
102103012	Biotechnology	Cell Biology	Dr. Sanjukta Patra	Web	IIT Guwahati	Select Prokaryotic and Eukaryotic cells Plant and animal cells Principles of membrane organization, membrane proteins Cytoskeletal elements and architecture Structure and function of cytoplasm, nucleus and mitochondria Ribosome, endoplasmic reticulum, golgi bodies and lysosomes Peroxisomes, chloroplast and vacuoles Extracellular matrix Cell locomotion (amoeboid, flagella, cillar) Types of cell division, Cell cycle and molecules that control cell cycle Mitosis Meiosis Genetic material in a cell Human Cromosome Giant chromosomes Transport across cell membrane Lactose permease Lysosomal membrane ATP dependent proton pumps Cotransport: Symport, Antiport Transport in prokaryotic cells Endocytosis and Exocytosis Entry of virus in cells Entry of toxins into the cells Hormone Plant growth factors Plant hormones Cell signaling Receptor Cyclic adenosine monophosphate ( cAMP) Cyclic Guanosine monophosphate Calcium ion flux and its role in cell signaling G � Protein Cell Culture Characterization of Cells Contamination Differentiation Three Dimentional Culture Role of matrix in cell growth Components of blood plasma cell Haemopoiesis
102103015	Biotechnology	Microbiology	Dr. Gurvinder Kaur Saini	Web	IIT Guwahati	Select History of Microbiology Members of the microbial world, scope, relevance and future of microbiology The prokaryotic cell, size, shape and arrangement of bacterial cells Structures internal to the cell wall Structures external to the cell wall Microscopes I Microscopes II Preparation and staining of specimens, simple stains, differential and special stains Microbial nutrient requirements and nutritional types of microorganisms Uptake of nutrients by the cell Culture media, Isolation and cultivation of pure cultures Growth of bacterial cultures, growth curve and measurement of microbial growth Influence of Environmental factors on microbial growth The use of physical methods in Microbial control The use of chemical methods in Microbial control Antimicrobial Chemotherapy Overview of microbial metabolism Carbohydrate Catabolism Anaerobic respiration and Fermentation Protein and Lipid Catabolism Photosynthesis Biosynthesis of Amino acids and Lipids Biosynthesis of Pyramidines Biosynthesis of Purines and Peptidoglycan Structure and function of genetic material DNA replication The genetic code and the gene structure The expression of genes Regulation of Genes DNA Repair and Microbial Recombination Bacterial Plasmids and Transposable elements Mutations and their chemical basis, detection and isolation of mutants Bacterial Conjugation,Transduction and Transformation Microbial Taxonomy and Phylogeny Archaea and Bacteria Fungi, algae, protozoa and slime molds Viruses- Introduction and General Characteristics The Bacteriophages The Viruses of Eukaryotes Microbial Pathogenicity and Diseases Study Questions 1 Study Questions 2 Study Questions 3 Study Questions 4 Study Questions 5 Study Questions 6 Study Questions 7 Study Questions 8 Study Questions 9 Study Questions 10
102103016	Biotechnology	Plant Biotechnology	Dr. Rakhi Chaturvedi	Web	IIT Guwahati	Select Introduction to plant tissue culture Lab facilities and operations Tissue Culture Media Plant Tissue culture techniques Micropropagation by adventitious shoot proliferation Micropropagation by Axillary shoot proliferation Meristem culture for virus elimination Somatic Embryogenesis and Artificial seed production Doubled Haploid production Triploid production by Endosperm culture Cell suspension cultures Protoplast Isolation and Regeneration Somatic Hybridization and Cybridization Somaclonal, Protoclonal and Gametoclonal variation Cryopreservation Cytological and various staining procedures for Ploidy analysis Microscopy Electron Microscopy Flow Cytometry and cell sorting Plant Histological Techniques Genetic material of plant cells Restriction Enzymes Plant transformation and transformation vectors Mode of Gene delivery in plant Agrobacterium mediated Gene transfer Ti and Ri Plasmids Selection and screening of transformations PCR and Hybridization technique Gene silencing Applications of Genetic Engineering Advantages of Plant cell, Tissue and organ culture as source of secondary metabolites Hairy Root cultures Screening of High yielding cell lines and extraction of High value Industrial Products Fractionation and Bioassays of plant extract Growth and Production Kinetics of Cell cultures in Shake Flasks Bioreactors for Plant Engineering Manipulation in Production Profile by Abiotic and Biotic Elicitation Biotransformation Aims Scope and Bottlenecks of Molecular Farming Production of Industrial Enzymes and Biodegradable Plastics Production of Antibodies Metabolic Engineering for Production of Fatty Acids, Industrial Oils, Terpenoids and Flavonoids
102103017	Biotechnology	Proteomics and Genomics	Dr. Vikash Kumar Dubey	Web	IIT Guwahati	Select Introduction and Scope of Proteomics Protein Separation Techniques: General Concepts of Chromatography Ion Exchange Chromatography-I Ion Exchange Chromatography �II Size Exclusion (Gel Filtration) Chromatography Affinity Chromatography-I Affinity Chromatography-II Affinity Chromatography-III Polyacrylamide Gel Electrophoresis Polyacrylamide Gel Electrophoresis-II Isoelectric Focusing (IEF) Two Dimensional PAGE for Proteome Analysis Analysis of 2D Gels Suggested Reading and Home Assignment Introduction to Mass Spectrometry-I Introduction to Mass Spectrometry-II Strategies for Protein Identification Protein Sequencing Protein Modifications and Proteomics Protein-Protein Interaction Midterm Examination Protein Engineering Clinical and Biomedical Applications of Proteomics Proteomics Industry Protein Chip Technology Functional Proteomics and Proteome Database Overview of Deoxyribonucleic Acid (DNA) and Ribonucleic Acid (RNA) Structure Agarose Gel Electrophoresis for DNA Analysis Methods of Preparing Genomic DNA DNA Sequence Analysis Methods-I DNA Sequence Analysis Methods-II Home Assignment and Further Reading Gene Variations and Single Nucleotide Polymorphism Expressed Sequence Tags DNA & Disease Association Basics of DNA Cloning-I Basics of DNA Cloning-II Polymerase Chain Reaction DNA Fingerprinting Human Genome Project Final Examination
102103038	Biotechnology	Cellular and Molecular Immunology	Dr. Sachin Kumar	Web	IIT Guwahati	Select Introduction Properties of Immune system Innate immune system (Part I) Innate immune system (Part II). Adaptive immune system (Part I) Questions: Introduction Antibodies and Antigens (Part I) Antibodies and Antigens (PartII) Major histocompatability complex (Part I) Major histocompatability complex (Part II) Antigen processing and presentation to T lymphocyte (Part I) Antigen processing and presentation to T lymphocyte (Part II) Antigen receptors and accessory molecules of T lymphocytes. (Part I) Antigen receptors and accessory molecules of T lymphocytes. (Part II) Development of Lymphocytes (Part I) Development of Lymphocytes (Part II) Activation of Lymphocytes (Part I) Activation of Lymphocytes (Part II) B cell activation and antibody production (Part I) B cell activation and antibody production (Part II) Immune memory response (Part I). Immune memory response (Part II). Cytokines (Part I Cytokines (Part II) Mechanism of cell mediated immune response (Part I) Mechanism of cell mediated immune response (Part II) Mechanism of antibody medicated immune response (Part I) Mechanism of antibody medicated immune response (Part II) Immunity to microbes (Part I) Immunity to microbes (Part II) Transplant immunology (Part I) Transplant immunology (Part II) Tumor immunology (Part I) Tumor immunology (Part II) Hypersensitivity (Part I) Hypersensitivity (Part II) Congenital and acquired immunodeficiency (Part I) Congenital and acquired immunodeficiency (Part II). Laboratory techniques commonly used in immunology (Part I) Laboratory techniques commonly used in immunology (Part II) Adaptive immune system (Part II) Questions: Antibodies and Antigens Questions: Development of immune cells Questions: Mechanism of immune response Questions: Immunity to pathogens Questions: Hypersensitivity and immunodeficiency Mid Term Assignment Final Assignment
102103039	Biotechnology	General Virology	Dr. Sachin Kumar	Web	IIT Guwahati	Select General Concepts: Virus history General Concepts: Virus Diversity General Concepts: Virus Shapes General Concepts: Virus Sizes General Concepts: Components of genomes. Isolation and purification of viruses and components. Questions : General Concepts Consequences of virus infection to animals and human (Part I). Consequences of virus infection to animals and human (PartII). Viral infection: affect on host macromolecules (Part I). Viral infection: affect on host macromolecules (Part II). Viral infection: establishment of the antiviral state (Part I). Viral infection: establishment of the antiviral state (Part II). Viruses counter attack mechanisms (Part I). Viruses counter attack mechanisms (Part II) Classification of viruses and nomenclatures (Part I). Classification of viruses and nomenclatures (Part II) Positive strand RNA viruses Picornaviruses. Flaviviruses- West Nile virus Flaviviruses- Dengue virus Coronaviruses SARS pathogenesis Negative strand RNA viruses Paramyxoviruses Orthomyxoviruses Influenza pathogenesis and Bird flu Rhabdoviruses Rabies pathogenesis dsRNA viruses Reoviruses Questions: Virus host interaction Questions: Positive strand RNA virus Questions: Negative strand RNA viruses Retroviruses: structure, classification, life cycle Reverse transcription HIV- viral pathogenesis AIDS Questions: Other RNA viruses Small DNA viruses: parvo- and polyomaviruses Large DNA viruses Herpesviruses Adenoviruses Poxviruses Miscellaneous viruses Questions: DNA viruses Mid Term Assignment Final Assignment
102103041	Biotechnology	Gene Therapy	Dr. Sachin Kumar	Web	IIT Guwahati	Select Introduction (part I) Introduction (part II) Somatic and germ line gene therapy (part I) Somatic and germ line gene therapy (part II) Gene replacement and gene addition (part I) Gene replacement and gene addition (part II) In vivo, ex vivo and in vitro gene therapy (Part I) In vivo, ex vivo and in vitro gene therapy (PartII) Transgenic animal models (Part I) Transgenic animal models (Part II). Vehicles for gene transfer-viral vectors: retrovirus (Part I). Vehicles for gene transfer-viral vectors: retrovirus (Part II). Adenovirus Adeno-associated virus. Lentivirus Recombinant SV40 virus Nonviral vectors DNA vaccines Liposomes and lipoplexes (Part I). Liposomes and lipoplexes (Part II). Naked DNA Transposon Cancer gene therapy (Part I). Cancer gene therapy (Part II). Cancer gene therapy (Part III). RNA-DNA chimera Gene therapies for Criglar-Najjar syndrome (Part I). Gene therapies for Criglar-Najjar syndrome (Part II). Cystic fibrosis (part I) Cystic fibrosis (part II) Duchnne muscular dystrophy Bleeding disorders (part I) Bleeding disorders (part II) Tyrosinemia Severe combined immunodeficiency syndrome (SCID) (part I) Severe combined immunodeficiency syndrome (SCID) (part II) Gene therapy of non-heritable disorders (part I) Gene therapy of non-heritable disorders (part II) Recent advancement in Gene Therapy (part I) Recent advancement in Gene Therapy (part II) Mid Sem Exam Final Sem Exam Question Set I Question Set II Question Set III Question Set IV Question Set V Question Set VI
102103044	Biotechnology	Bioanalytical Techniques and Bioinformatics	Dr. Nitin Chaudhary,Dr. Vishal Trivedi	Web	IIT Guwahati	Select Introduction (part I) Introduction (part II) Somatic and germ line gene therapy (part I) Somatic and germ line gene therapy (part II) Gene replacement and gene addition (part I) Gene replacement and gene addition (part II) In vivo, ex vivo and in vitro gene therapy (Part I) In vivo, ex vivo and in vitro gene therapy (PartII) Transgenic animal models (Part I) Transgenic animal models (Part II). Vehicles for gene transfer-viral vectors: retrovirus (Part I). Vehicles for gene transfer-viral vectors: retrovirus (Part II). Adenovirus Adeno-associated virus. Lentivirus Recombinant SV40 virus Nonviral vectors DNA vaccines Liposomes and lipoplexes (Part I). Liposomes and lipoplexes (Part II). Naked DNA Transposon Cancer gene therapy (Part I). Cancer gene therapy (Part II). Cancer gene therapy (Part III). RNA-DNA chimera Gene therapies for Criglar-Najjar syndrome (Part I). Gene therapies for Criglar-Najjar syndrome (Part II). Cystic fibrosis (part I) Cystic fibrosis (part II) Duchnne muscular dystrophy Bleeding disorders (part I) Bleeding disorders (part II) Tyrosinemia Severe combined immunodeficiency syndrome (SCID) (part I) Severe combined immunodeficiency syndrome (SCID) (part II) Gene therapy of non-heritable disorders (part I) Gene therapy of non-heritable disorders (part II) Recent advancement in Gene Therapy (part I) Recent advancement in Gene Therapy (part II) Mid Sem Exam Final Sem Exam Question Set I Question Set II Question Set III Question Set IV Question Set V Question Set VI
102103045	Biotechnology	Fundamentals of Biotechnology	Dr. Vishal Trivedi	Web	IIT Guwahati	Select Introduction Water properties and its role in controlling biochemical reactions Cellular structure: prokaryotcic and eukaryotic cells (Part-I) Cellular structure: prokaryotcic and eukaryotic cells(Part-II) Metabolic reactions (Part-I) Metabolic reactions (Part-II) Metabolic reactions (Part-III) Growth media for different expression system Microbial growth kinetics DNA Libaries (PART-I) DNA Libaries (PART-II) Identification and Isolation of a particular gene Basics of Cloning (Part-I) Basics of Cloning (Part II) Polymerase chain reaction (Part-I) Polymerase chain reaction (Part-II) Cloning Vector (Part-I) Cloning Vector (Part-II) Cloning Vector (Part-III) DNA Delivery in Host Cell (Part-I) DNA Delivery in Host Cell (Part-II) Screening of recombinant clone (Part-I) Screening of recombinant clone (Part II) Protein Production strategies in Expression System (Part-I) Protein Production strategies in Expression System (Part-II) Protein Production strategies in Expression System (Part-III) Protein Production strategies in Expression System (Part-VI) Product Recovery from over-expressed cells Basics of Chromatography Ion-Exchange chromatography Hydrophobic Interaction chromatography Gel Filtration chromatography Affinity Chromatography Thin Layer Chromatography Electrophoresis (Part-I) Electrophoresis (Part-II) DNA sequencing Protein sequencing Biotechnology Application-I Biotechnology Application-II Mid Sem Exam End Sem Exam
102103047	Biotechnology	Experimental Biotechnology	Dr. Vishal Trivedi, Dr. Nitin Chaudhary	Web	IIT Guwahati	Select Introduction Water and aqueous solutions Buffers Determination of protein concentration by ultraviolet spectroscopy Determination of protein concentration by Bradford method Estimation of DNA using diphenylamine method Melting temperature of DNA Equilibrium unfolding of protein Circular dichroism of proteins � I Circular dichroism of proteins � II Fourier transform infrared spectroscopy of proteins Enzyme activity Electrophoresis (Part-I) Staining of PAGE Gels Western Blotting Electrophoresis (Part-II) Horizontal Gel Electrohpresis RNA gel Nucleic acid Detection Introduction to chromatography Thin Layer Chromatography Packing a gel filtration column and determination of void volume Protein molecular weight determination using gel filtration chromatography Protein purification using ion exchange chromatography Protein purification using metal chelate affinity (Ni-NTA) chromatography HPLC of proteins and peptides Antibody (Part-I) Antibody (Part-II) Purification of antibody ELISA Light Microscopy Microscopy-II Microscopy (Part-III) Electron Microscopy-I Electron Microscopy-II Atomic Force Microscopy Preparation of Competent Cells Transformation of Competent Cells Isolation of Recombinant Clones Analysis and Verification of Recombinant Plasmids Mid Semester End Semester |
102104042	Biotechnology	Animal Physiology	Prof. Mainak Das	Video	IIT Kanpur	Select Lecture-01-Animal Physiology Lecture-02-Animal Physiology Lecture-03-Animal Physiology Lecture-04-Animal Physiology Lecture-05-Animal Physiology Lecture-06-Animal Physiology Lecture-07-Animal Physiology Lecture-08-Animal Physiology Lecture-09-Animal Physiology Lecture-10-Animal Physiology Lecture-11-Animal Physiology Lecture-12-Animal Physiology Lecture-13-Animal Physiology Lecture-14-Animal Physiology Lecture-15-Animal Physiology Lecture-16-Animal Physiology Lecture-17-Animal Physiology Lecture-18-Animal Physiology Lecture-19-Animal Physiology Lecture-20-Animal Physiology Lecture-21-Animal Physiology Lecture-22-Animal Physiology Lecture-23-Animal Physiology Lecture-24-Animal Physiology Lecture-25-Animal Physiology Lecture-26-Animal Physiology Lecture-27-Animal Physiology Lecture-28-Animal Physiology Lecture-29-Animal Physiology Lecture-30-Animal Physiology Lecture-31-Animal Physiology Lecture-32-Animal Physiology Lecture-33-Animal Physiology Lecture-34-Animal Physiology Lecture-35-Animal Physiology Lecture-36-Animal Physiology Lecture-37-Animal Physiology Lecture-38-Animal Physiology Lecture-39-Animal Physiology Lecture-40-Animal Physiology
102106022	Biotechnology	Downstream Processing	Prof. Mukesh Doble	Video	IIT Madras	Select Introduction Mass Balance, Heat Balance, Flow sheet Costing Costing (continued) Physical and Chemical Principles in Down Stream Problems in Mass Balance, Flow sheet Cell Breakage Cell Breakage (Continued) Solid Liquid Separation Solid Liquid Separation (Continued) Solid Liquid Separation - Problems Pre-Treatment and Filters Adsorption Adsorption (Continued) Adsorption (Continued) Adsorption. (Continued) Liquid Liquid Extraction Liquid Liquid Extraction (Continued) Liquid Liquid Extraction (Continued) Liquid Liquid Extraction(Continued) Reversed Micellar and Aqueous Two Phase Extraction Membranes Membranes (Continued) Membranes (Continued) Membranes(Continued) Precipitation Chromatography Chromatography (Continued) Chromatography (Continued) Chromatography (Continued) Chromatography(Continued) Chromatography ( Continued) Chromatography (Continued ) HPLC HPLC. Crystallisation Drying Drying and Distillation Stabilisation, Utilities and Other Auxiliary Processes And Absorption Absorption, Electrophoresis/SDS PAGE Future Trends, Summary of Course
102106023	Biotechnology	Introduction to Computational Neuroscience	Dr. V Srinivasa Chakravarthy	Web	IIT Madras	Select Introduction Neuron: Structure and Function Mathematical Preliminaries Hodgkin-Huxley Model Modeling the neuron components Simplified Neuron Models Neural Networks Neural Networks- Hopfield network Unsupervised Learning-Hebbian Learning Unsupervised Learning-Competitive Learning
102106025	Biotechnology	Molecular Cell Biology	Prof. Devarajan Karunagaran	Web	IIT Madras	Select Introduction to Molecular Cell Biology, Life and Evolution Eukaryotes Nuclear Structure Chromatin Packing Microtubules Micro and Intermediate Filaments Cell Membrane and Transport Mechanisms Across The Cell Membrane Microvilli, Gap Junctions and Inter Cellular Communication Endoplasmic Reticulum, Lysosomes, Golgi Apparatus and Endocytosis Microscopy Methods to Manipulate Cells and Proteins Cell Division Cell Cycle Cell Growth and Differentiation mTOR Pathway Apoptosis/Programmed Cell Death Introduction to Signals and Signal Transduction GPCR Signaling TGF-β Signaling Wnt Signaling NF-κB Signaling Notch Signaling RTK Signaling Ras-MAPK Signaling Akt/PKB Signaling JAK-STAT Signaling Ion Channels Hedgehog Signaling Cell-Cell Interaction Introduction to Cancer Biology Genes Associated with Cancer Deregulated Signaling Pathways in Cancer Tumor Immunology Molecularly Targeted Therapies for Cancer Epigenetics and Cancer DNA Repair Mechanisms and Cancer An Introduction to Pathogens Bacterial Pathogenesis Viral Pathogenesis Protozoan Disease-Malaria
102106026	Biotechnology	Thermodynamics	Prof. G.K. Suraishkumar	Video	IIT Madras	Select Introduction and Review Need for Analysis Additional Thermodynamic Functions State and Path Variables Equations for a Closed system Chemical Potential Concept Gibbs-Duhem Equation Maxwell�s relations Inter-Relationships between Thermodynamic Variables Some Useful Mathematical Manipulations Thermodynamic Relations for a Closed System with 1 mole of a pure Substances Maximum Work,Lost Work Review of Closed Systems Open Systems Equations of State - Virial Equations Equations of State - Cubic Equations Volume Estimation Volume Estimation (cont.,)Generalized correlations Generalized correlations(cont)Residual Properties Residual Properties(cont.,) Generalized Correlations and Residual Properties Fugacity Coefficient Estimation Review of Module 3 Learning Aspects Chemical Potential Formulations Lewis and Randall rule partial Molar Properties Partial Molar Property Estimation from Mixing Experiments Partial Molar Property Estimation (cont.,) Excess Property Activity Coefficient from Excess Property Activity Coefficient from Excess Property (cont.,) Activity Coefficient from Excess Property(cont.,) Models for Activity Coefficient in Binary Systems Models for Activity Coefficient in Binary Systems(cont.,) Review of Module 4 Criteria for Phase Equilibrium Phase Rule for Non-reacting Biosystems Clausius - Clayperon Equation Clausius - Clayperon Equation(cont.,)vapour-Liquid Equilibrium Vapour-Liquid Equilibrium(cont.,) Estimation of Fugacity coefficient from Equilibrium P-V-T Data Liquid/Liquid and Solid/Liquid Equilibria Review of Module 5 Criteria for Bio-reaction Equilibria Phase rule for Reacting Biosystems Equilibrium constants Effect of Temperature and Pressure on the Equilibrium constants Reaction in Liquid or Solid Phases Free energy Changes for some Bioreactions Electrolytes Course Review
102106035	Biotechnology	Systems Biology	Dr. M. Vijayalakshmi	Web	IIT Madras	Select Systems Biology � An Overview Overview of Gene Control Gene Control Mechanisms at Transcriptional and Translational Levels Genetic Switches � I Genetic Switches � II Biochemical, Genetics and Systems Biology Paradigms Quorum Sensing � The Language of Bacteria Quorum Sensing in Gram Negative and Gram Positive Bacteria Hybrid Quorum Sensing in Vibrio harveyi � Two Component Signalling Programmed Population Control by Cell �Cell Communication and Regulated Killing � I Programmed Population Control by Cell �Cell Communication and Regulated Killing � II Drosophila melanogaster � Life Cycle Drosophila melanogaster � Morphogen Gradient Establishment of Developmental Precision and Proportions in the Early Drosophila Embryo Michaelis Menten Kinetics � Enzyme Binding and Cooperativity Michaelis Menten Kinetics � I Michaelis Menten Kinetics � Enzyme Inhibition Michaelis Menten Kinetics � Identical Independent Binding Sites Michaelis Menten Kinetics � Sequential Model of Cooperativity Michaelis Menten Kinetics , Non Identical Interacting Binding Sites Structure ad Genetic Map of Lambda Phage The Classical Genetic Switch in Lambda Phage - Lytic and Lysogeny Decisions Noise in Gene Expression Quantifying Noise in Gene Expression Noise in Gene Regulatory Networks Noise based Switches and Amplifiers for Gene Expression Bacterial Chemo taxis The Signalling Pathway in Chemo taxis Gene Expression Networks Transcription Networks I Transcription Networks II Networks in Biology - Network Motifs Feed Forward Loops in Biological Systems Coherent Feed Forward Loops Incoherent Feed Forward Loops Kinetics of Coherent FFLs-Sign sensitive delay circuits Kinetics of Incoherent FFLs-Sign sensitive accelerator circuits Pathway Mapping through KEGG Cytoscape Virtual Cell
102103013	Biotechnology	Genetic Engineering & Applications	Dr. Utpal Bora	Web	IIT Guwahati	Select Role of genes within cells, genetic code, genetic elements that control gene expression Method of creating recombinant DNA molecules Types, biology and salient features of vectors in recombinant DNA technology: Plasmids Types, biology and salient features of vectors in recombinant DNA technology Safety guidelines for recombinant DNA research Control of spills and mechanism of implementation of biosafety guidelines Enzymes in genetic engineering: Restriction nucleases: exo & endo nucleases Enzymes in modification- Polynucleotide phosphorylase, DNase and their mechanism of action. Enzymes in modification- Methylases and phosphatases and their mechanism of action. Enzymes in modification- Polynucleotide kinase, Ligases, RNase and their mechanism of action. Methods of nucleic acid detection. Polymerase chain reaction (PCR) and its applications Variations in PCR and their applications Methods of nucleic acid hybridization Probe and target sequences Nucleic acid mutagenesis in vivo and in vitro Isolation and purification of nucleic acid (genomic/plasmid DNA and RNA) Quantification and storage of nucleic acids. Construction of cDNA library Construction of Genomic library Screening and preservation of DNA libraries Gene transfer techniques: biological methods Gene transfer techniques: chemical methods Gene transfer techniques: physical or mechanical methods Agro- bacterium mediated gene transfer in plants Chloroplast transformation Transgenic science in plant improvement Biopharming - plants as bioreactors Transgenic science for animal improvement Biopharming- Animals as bioreactor for recombinant protein Gene mapping in plants and animals Marker-assisted selection for plant breeding and livestock improvement Microbial biotechnology: Genetic manipulation Engineering microbes for the production of antibiotics and enzymes Engineering microbes for the production of insulin, growth hormones, monoclonal antibodies Engineering microbes for clearing oil spills Gene therapy: Introduction and Methods Gene targeting & silencing Gene therapy in the treatment of diseases Challenges & future of gene therapy M1-Problems M2-Problems M3-Problems M5-Problems M6-Problems M7-Problems M8-Problems DNA Sequencing and Cloning Stategies M4-Problems
102106036	Biotechnology	Tissue Engineering	Dr. S. Swaminathan	Web	SASTRA University	Select Introduction to Tissue Engineering Cells as Therapeutic Agents Cells as Therapeutic Agents. Tissue Organization Extracellular Matrix Tissue Organization. Cellular Fate Processes Cellular Fate Processes. Cell Division Cell death – biological process of apoptosis Coordination of cellular fate process Coordination of cellular fate process. Malfunctions in soluble signalling How Cell-Extra cellular matrix interactions can co-ordinate cell fates? Direct cell-cell contact Bidirectional nature of cell-cell communication Microscopic Characterization of Tissues and Biomaterials Basic tools to detect cell fate and cell functions Cell and Tissue Culture Culture Environment and maintenance of cells in vitro Cell separation Biomaterials in tissue engineering Scanning Electron Microscope Biomaterials in tissue engineering. Scaffold fabrication technologies and tailoring Biomaterials Growth factor delivery Stem cells Stem cells and tissue engineering Gene transfer Gene transfer. Gene transfer.. Bioreactors in tissue engineering Artificial blood vessels Artificial blood vessels. Artificial pancreas Artificial Pancreas. Liver Tissue Engineering
102107028	Biotechnology	Analytical Technologies in Biotechnology	Dr. Ashwani K. Sharma	Video	IIT Roorkee	Select Basic concepts in microscopy 1 Basic concepts in microscopy 2 Dark-field and phase contrast microscopy Differential interference contrast and polarization Fluorescence and confocal microscopy Transmission electron microscopy Transmission electron microscopy cont. and scanning electron microscopy Basic concepts 1 Basic concepts 2 GM counting and Scintillation counting Scintillation counting continued Autoradiography and RIA Safety aspects and applications Introduction and Basic concepts in chromatography 1 Basic concepts in chromatography 2 Low-pressure liquid chromatography (LPLC) and high performance liquid chromatography (HPLC) Ion-exchange chromatography Gel-filtration chromatography Affinity chromatography Gas-liquid chromatography Basic concepts in electrophoresis Horizontal and vertical gel electrophoresis Native gel electrophoresis and SDS-PAGE Isoelectric focusing (IEF), 2-D gel electrophoresis and protein detection methods Electrophoresis of nucleic acids Immunoelectrophoresis and capillary electrophoresis Introduction and basic concepts 1 Basic concepts-2 Types of centrifuges and analytical ultracentrifugation method Separation methods in preparative ultracentrifuges Types of rotors Types of rotors cont. and care of rotors Introduction and basic concepts UV-Visible spectroscopy Infrared and fluorescence spectroscopy Circular dichroism (CD) spectroscopy Nuclear magnetic resonance (NMR) spectroscopy and X-ray crystallography Atomic spectroscopy and mass spectrometry Polymerase chain reaction(PCR) DNA sequencing methods Enzyme linked immunosorbent assay (ELISA)
102106048	Biotechnology	NOC:Principles of Downstream techniques in Bioprocess	Prof. Mukesh Doble	Video	IIT Madras	Select Introduction Mass balance, Heat Balance, Flow sheet Costing Cell Breakage Solid Liquid Separation Pre-treatment and Filters/centrifuge Liquid-Liquid Extraction Liquid-Liquid extraction (continued) Adsorption Reversed micellar and aqueous two phase extraction Membranes Membranes (continued) Product stabilization, Drying, Lyophilisation Precipitation and crystallization Electrophoresis / SDS PAGE Chromatography Chromatography continued_1 Chromatography continued_2 Chromatography continued_3 Future trends, Other downstream operations/Summary of the course
102101049	Biotechnology	NOC:Proteins and Gel-Based Proteomics	Prof. Sanjeeva Srivastava	Video	IIT Bombay	Select Proteins and Gel-Based Proteomics; Course Introduction L1. Introduction to amino acids L2. Introduction to proteins L3. Protein folding & misfolding L4. Protein purification techniques L5. Introduction to proteomics L6. Systems biology and proteomics L7. Sample preparation and pre-analytical factors L8. Sample preparation: Pre-analytical factors (contd.) L9. Sample preparation: Protein extraction and quantification L10. One-dimensional electrophoresis L11. 2-DE: Rehydration, IEF & Equilibration L12. 2-DE: Second dimension, staining & destaining L13. 2-DE: Gel analysis L14. 2-DE: Applications L15. 2-DE: Applications (contd.) & Challenges L16. 2D-DIGE: Basics L17. 2D-DIGE: Data analysis L18. 2D-DIGE: Applications L19. Protein identification using MALDI-TOF/TOF L20. Proteomics experiment data analysis & challenges
102101050	Biotechnology	NOC:Mass spectrometry based proteomics	Prof. Sanjeeva Srivastava	Video	IIT Bombay	Select Introductory lecture Lecture 1: Introduction to proteomics Lecture 2: Proteomics and sample preparation Lecture 3: Bacterial protein extraction Lecture 4: In-gel digestion Lecture 5: Fundamentals of mass spectrometry Lecture 6: Chromatography technologies Lecture 7: Liquid chromatography Lecture 8: Mass spectrometry: Ionization sources Lecture 9: Mass spectrometry: Mass analyzers Lecture 10: MALDI sample preparation and analysis Lecture 11: Introduction to quantitative proteomics Lecture 12: Hybrid mass spectrometry configurations Lecture 13: SILAC: In Vivo labeling Lecture 14: iTRAQ: In Vitro labeling Lecture 15: TMT: In vitro labeling Lec 16: Quantitative proteomics data analysis Lec 17: Proteomics and Systems biology I Lec 18: Proteomics & Systems biology II Lec 19: Proteomics applications Lec 20: Advances and challenges in proteomics